EVALUATION IN VITRO DE L’ACTIVITE IMMUNO-MODULATRICE DES EXTRAITS HYDRO-ETHANOLIQUE ET AQUEUX DES FEUILLES DE ANNONA MURICATA ET ESSAI DE MISE EN FORME GALENIQUE

LOGMO MAHI (maybrune@yahoo.fr)
Hematology, Microbiology, Parasitology, Immunology and infectious diseases, University of Yaounde I
June, 2016
 

Abstract

INTRODUCTION
Annona muricata is a plant of the Annonaceae family widely used by people in many countries of the world, especially those of Cameroon. Both cultivated and wild, this plant is presented as having antimicrobial, anti-cancer, anti-hypertensive, antidiabetic properties and others.

OBJECTIVE
Evaluate in vitro the action of hydro-ethanolic and aqueous leaves extracts of Annona muricata on immune system cells to achieve drug formulation test.

MATERIAL AND METHOD
The study of phytochemical composition of the extracts was performed using colorimetric reactions while the content of total phenolic compounds in the extracts was determined by the method of Folin-Ciocalteu. The extracts antioxidant power was evaluated by DPPH (qualitative and quantitative) and FRAP methods. The immunomodulatory activity was assessed using PBMCs and a set of seven (07) cytokines (IL-17A, IFN γ, TNFα, IL-10, IL-6, IL-4, IL-2). Drug formulation test was performed using the monographs of the European Pharmacopoeia 8.0 on the preparation and quality control of Capsules.

RESULTS
The study of phytochemical composition revealed the presence of phenolics compounds, cardiac glycosides and sugars in both extracts, while alkaloids were present only in the hydro-ethanolic extract. The dosage of polyphenols showed that at the concentration of 1mg/mL, polyphenols content of hydro-ethanolic extract was higher than that of aqueous extract (p value <0.05). The results of DPPH have found that at the same concentrations, the inhibition percentage of hydro-ethanolic extract was higher than that of aqueous extract. Statistical analysis confirmed that antiradical power (AP) of extracts were significantly different (p <0.05) and, significantly lower than those of ascorbic acid (p-value <0.05). It appears from the FRAP test that hydro-ethanolic extract has higher reducing power than aqueous extract (p <0.05). The results of immuno-modulatory activity showed that at 1, 10, 100 and 1000µg, both extracts positively influence cytokine production. In general, the best profiles were obtained with the aqueous extract and cytokines that are most expressed are in order of importance IL-6, TNFα and IL-10. However, The hydro-éthanolic extract better influenced the production of IL-17A. The drug formulation test was carried out with the aqueous extract. Thus, from 87.5g extract, we make 350 capsules of 250 mg each. These capsules have satisfied to the mass uniformity and desintegration tests.

CONCLUSION
Given the results of this study, it appears that hydro-ethanolic and aqueous extracts of Annona muricata leaves are potential sources of antioxidants and immuno-modulators compounds. It’s therefore easy to understand why this plant is widely used in traditional medicine in treating many infectious and non-infectious diseases.


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